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Image Search Results
Journal: Neural Regeneration Research
Article Title: Upregulation of circ0000381 attenuates microglial/macrophage pyroptosis after spinal cord injury
doi: 10.4103/1673-5374.386399
Figure Lengend Snippet: Characterization of SCI rats. (A) Experimental procedures. (B) Representative images and quantitative analysis of H&E stained spinal cord at 7 days after SCI or sham surgery. After SCI, there was a larger spinal cord cavity compared with sham-operated animals. Scale bars: 500 µm. (C) BBB locomotion scores on days 1–42 after SCI or sham surgery. Data are presented as mean ± SEM, n = 6/group. *** P < 0.001, Student’s t -test (B) or two-way mixed model analysis of variance followed by Tukey’s post hoc analysis (C). BBB: Basso-Beattie-Bresnahan; ELISA: enzyme-linked immunosorbent assay; H&E: hematoxylin and eosin; IF: immunofluorescence; PCR: polymerase chain reaction; SCI: spinal cord injury; WB: western blot.
Article Snippet: According to the manufacturer’s instructions, cytokine levels were measured by enzyme-linked
Techniques: Staining, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Polymerase Chain Reaction, Western Blot
Journal: Neural Regeneration Research
Article Title: Upregulation of circ0000381 attenuates microglial/macrophage pyroptosis after spinal cord injury
doi: 10.4103/1673-5374.386399
Figure Lengend Snippet: SCI induces cell pyroptosis in the spinal cord. (A) mRNA levels of NLRP3, caspase-1, GSDMD, IL-1β, and IL-18 in the spinal cord were measured by real-time polymerase chain reaction on day 7 after SCI or sham surgery. (B) Representative western blots and quantification of NLRP3, caspase-1, and GSDMD protein levels in the spinal cord on day 7 after SCI or sham surgery. (C) Concentrations of IL-1β and IL-18 in the spinal cord were measured by enzyme-linked immunosorbent assay on day 7 after SCI or sham surgery ( n = 6/group). Data are presented as mean ± SEM ( n = 6/group). *** P < 0.001, vs . sham group (Student’s t -test). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; GSDMD: gasdermin D; IL: interleukin; mRNA: messenger RNA; NLRP3: NOD-like receptor 3; SCI: spinal cord injury.
Article Snippet: According to the manufacturer’s instructions, cytokine levels were measured by enzyme-linked
Techniques: Real-time Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay